Uplcqtofmsbased phenolic profiling of melastomaceae, their. Comparison of drug detection by three quadrupole timeof. Separation was carried out using acquity uplc hsst3 column 100. Ionbench with the waters xevo g2xs qtof mass spectrometer. How to rebuild the reference probe of a xevo or synapt tof ms. A hexapole collision cell, between the two mass analysers, can be used to induce.
For the scientist who needs to identify, quantify and confirm the broadest range of compounds in the most complex and challenging samples the xevo g2xs tof provides maximum robustness with no compromise in performance, the highest quality, most comprehensive qualitative and quantitative information. Follow the procedure in your instruments overview and maintenance guidefor example, in section 7. The xevo g2xs qtof mass spectrometer combines the new xs collision cell with the proven, signature technologies of tofmrm, stepwavetm and quantoftm to deliver optimum levels of robust sensitivity and selectivity, resulting in the detection of more lowlevel compounds than ever before. Uhplcqtof ms screening of pharmaceuticals and their. Overview to scale the picture below is a simulation at scale of the thermo tsq quantis ms system right side connected with a vanquish uhplc left side, installed on a ionbench ms mass spec bench or table the vacuum pump sogevac sv65bi is enclosed in the noise reduction cabinet, under your mass spectrometer thermo tsq quantis. Waters xevo g2 qtof xevo g2 qtof is the most sensitive, exactmass, quantitative and. Waters xevo g2xs qtof manuals manuals and user guides for waters xevo g2xs qtof. Xevo g2 qtof laboratory equipment pdf manual download. It delivers the integrat ed workflow benefits of engineered simplicity while incorporating the groundbreaking quantof technology of the synapt g2 system.
Squirt methanol into the hinge joint and manually work the hinge back and forth to free movement. Considerations specific to the xevo g2 s tof solventleakage hazard the source exhaust system is designed to be robust and leaktight. Intended use of the xevo g2 xs qtof waters designed the orthogonal acceleration, timeofflight xevo g2 xs qtof for use as a research tool to deliver authenticated mass measurement. The xevo g2xs tof combines stepwave ion optics with the xs collision cell, providing a significant increase in sensitivity with no reduction in selectivity. The bench or supporting surface must be vibration free. Waters acquity hclass uplc with pda and column manager. Tof mrm, on the synapt g2s hdms and xevo g2xs mass spectrometers. Overview to scale the picture below is a simulation at scale of the waters xevo g2 xs qtof lcms mass spectrometer system right side connected with an acquity uhplc left side, installed on a ionbench ms mass spec bench or table.
The analysis of the dichloromethane subfractions was performed on a waters xevo g2 qtof mass spectrometer waters ms technologies, manchester, uk, which was equipped with an electrospray ionization interface with waters acquity uhplc system waters, co. Please contact us for complete waters refurbishedused mass spectrometer system, warranty and service contract details. Use of different highresolution mass spectrometry platforms. The tof g2s sample kit 1 is intended for the verification of system performance for tof g2s. Uplcqtof msbased metabolomics and qrtpcr in enzyme. Comprehensive characterization of lignans from forsythia. Waters new xevo g2xs qtof mass spectrometer, bringing. The following table may help you calculate the additional btus of heat dissipation from this new equipment. Xevo source door is hard to open wkb52906 last updated. Ion mobility and liquid chromatographymass spectrometry. A waters acquity uplc system waters, milford, ma, usa was interfaced to a hybrid quadrupoleorthogonal accelerationtof mass spectrometer xevo g2 qtof, waters micromass, manchester, uk, using an orthogonal zsprayesi interface operating in both positive and negative ionisation modes. Metabolites of glibenclamide detected using %a %b 0 98 e2 5. Xevo g2 s tof instrument specifications 2 vacuum system differentially pumped, automated vacuum system comprising aircooled turbomolecular pumps and one backing pump either one rotary pump or one oil free pump.
This is designed for use with both gas and liquid sample inlet device, specifically a dedicated uplc and api gc. View and download waters xevo g2 qtof operators, overview and maintenance manual online. We have 1 waters xevo g2 xs qtof manual available for free pdf download. The xevo g2xs qtof is for research use only and is not intended for use in diagnostic applications. Vacuum read backs and system ventpump cycles are digitally monitored and controlled, to provide total software control and ensure failsafe operation in the event of power failure. The mz transmission range for a quadrupole in nonresolving mode is 20 to 32,000 mz for the 8000 mz quadrupole. The tof mass range is 20 to 100,000 mz in resolution mode, and 20 to 32,000 mz in high resolution mode. Featuring the new xs collision cell, the xevo g2xs qtof stands alone among its peers in offering. Jun 16, 2014 the xevo g2 xs qtof mass spectrometer combines the new xs collision cell with the proven, signature technologies of tof mrm, stepwavetm and quantoftm to deliver optimum levels of robust sensitivity and selectivity, resulting in the detection of more lowlevel compounds than ever before. Intact trastuzumab monoclonal antibody quantification. Vacuum read backs and system ventpump cycles are digitally monitored and controlled, to provide total software. A hexapole collision cell, between the two mass analysers, can be. Xevo g2xs tof performance, robustness, and flexibility made accessible to everyone.
Ms analysis was performed on a xevo g2 qtof waters ms technologies, manchester, uk. Our expert automation engineers will find, configure and offer the best devices for your application. We have 1 waters xevo g2xs qtof manual available for free pdf download. Xevo g2 tof delivers exceptional levels of sensitivity, selectivity, inspectrum dynamic range, speed of analysis, quantitative accuracy and exact mass. What is the latest version of masslynx software for the xevo g2 xs qtof and xevo g2 xs tof. During long analyses, critical hardware components stay clean for longer, so results are more reproducible and the laboratory stays productive. Quantitative performance attributes including sensitivity, accuracy. Xevo g2 xs tof is a benchtop time of flight ms combining stepwave ion optics, xs collision cell and quantof technology for superior robustness, sensitivity and selectivity, resulting in high quality, comprehensive qualitative and quantitative information. The xevo g2xs qtof combines stepwave ion optics, xs collision cell, and quantof technology for superior robustness, sensitivity, and selectivity along with dynamic range, mass accuracy, and speed of analysis, resulting in the highest quality, most comp rehensive qualitative and quantitative information. Waters introduces xevo g2xs qtof and xevo tqs micro mass. Jun 16, 2014 the xevo g2 xs qtof mass spectrometer combines the new xs collision cell with the proven, signature technologies of tof mrm, stepwave tm and quantof tm to deliver optimum levels of robust. In 2008, the present authors described an electromagnetostatic ems cell that can be retrofitted into virtually any existing type of mass spectrometer 16.
What is the latest version of masslynx software for the. How to rebuild the reference probe of a xevo or synapt tof. Sep 14, 2011 watch this xevo g2 qtof animation to learn how you can obtain the highest quality, most comprehensive information that will enable you to make the right decisions quickly and confidently. Uplcqtofmsbased phenolic profiling of melastomaceae. Qtof 2 users guide micromass uk limited floats road. Formation of 3 mcpd fatty acid esters from monostearoyl. Refurbished waters xevo g2 qtof pn 186005597 complete with esi source, pump and masslynx pc workstation. Waters xevo g2xs qtof overview and maintenance manual pdf. Intact quantification of trastuzumab is demonstrated using the xevo g2 xs qtof hrms and unifi scientific information system. Our expert automation engineers will find, configure and offer the best devices for. Your quantitative and qualitative questions answered.
The tof g2 s sample kit 1 is intended for the verification of system performance for tof g2 s. Analysis was performed using waters xevo g2s qtof and thermo qexactive mass spectrometers equipped with suitable uplc systems. Acquity uplc h class system coupled to a xevo g2 tof mass spectrometer waters corp. An acquity uplc iclass system coupled to a waters xevo g2xs qtof mass spectrometer detector waters uk. Wat introduces a new data acquisition mode, sonar, for its xevo g2xs quadrupole timeofflight qtof mass spectrometer.
Part 1 of 3 transforming high performance lcms analysis in. Nov 15, 2019 follow the procedure in your instruments overview and maintenance guidefor example, in section 7. Concerning the convenience of the xevo g2s qtof, i can say that the use of masslynx and targetlynx software provided by waters for targeted analysis was relatively practical and can afford a. This kit is designed for use by a waters certified engineer or user as part of a performance maintenance or qualification activity. Q3, qtof or orbitrap lcms which will be better for. Data were acquired in profile mode with masslynx software, version 4. In this report, the ems cell retrofitted into a hybrid q tof tandem.
Analysis was performed on a waters xevo g2xs qtof equipped with acquity uplci class and unifi software. For the scientist who needs to identify, quantify and confirm the broadest range of compounds in the most complex and challenging samples the xevo g2 xs tof provides maximum robustness with no compromise in performance, the highest quality, most comprehensive qualitative and quantitative information. Quantof technology delivers superior uplccompatible mass resolution, matrixtolerant dynamic range, quantitative. Xevo g2xs tof the xevo g2xs tof combines stepwave ion optics, xs collision cell, and quantof technology for superior robustness, sensitivity, and selectivity along with dynamic range, mass accuracy, and speed of analysis, resulting in the highest quality, most comprehensive qualitative and quantitative information. The proteus ecometer s is an ultrasonic liquid level sensor, battery powered and equipped with a digital radio transmitter. See the whole picture with the new 7250 gcqtof duration. In this report, the ems cell retrofitted into a hybrid qtof tandem. Request pdf quantitation by high resolution mass spectrometry. Waters advances proteomics and lipidomics research with novel. Intact trastuzumab monoclonal antibody quantification using. Uplcmse and quantof technology, offers unparalleled sensitivity with. Waters xevo g2s bench top qtof, designed for identification and confirmation of the broadest range of compounds in complex and challenging samples, is used for studying proteins and their complexes. Q tof 2 utilises a high performance, research grade quadrupole mass analyser, incorporating a prefilter assembly to protect the main analyser from contaminating deposits, and an orthogonal acceleration time of flight tof mass spectrometer. Analysis was performed using waters xevo g2 s q tof and thermo qexactive mass spectrometers equipped with suitable uplc systems.
The waters acquity uplc iclass ensures fast analysis with high resolution power. Antibody quantification using the xevo g2xs qtof high resolution mass. Intact trastuzumab monoclonal antibody quantification using the. The xevo g2xs qtof combines stepwave ion optics, xs. Waters recommends you perform a hazard analysis, assuming a maximum leak into the laboratory atmosphere of 10% lc eluate. For scientists who need to confidently identify and quantify unknown compounds in complex samples, the xevo g2 tof mass spectrometer with uplcmse and quantof technology offers exceptional levels of selectivity, inspectrum dynamic range, speed. The analysis of the dichloromethane subfractions was performed on a waters xevo g2 q tof mass spectrometer waters ms technologies, manchester, uk, which was equipped with an electrospray ionization interface with waters acquity uhplc system waters, co. The waters xevo g2 q tof is coupled to a waters acquity uplc iclass, that offers the most powerful solutions to complex separation challenges. The instrument incorporates the newest technologies in the area of ion optics for increased sensitivity.
Ionbench with the thermo tsq quantis mass spectrometer. Watch this xevo g2 qtof animation to learn how you can obtain the highest quality, most comprehensive information that will enable you to make the right decisions quickly and confidently. A waters acquity uplc system waters, milford, ma, usa was interfaced to a hybrid quadrupoleorthogonal acceleration tof mass spectrometer xevo g2 qtof, waters micromass, manchester, uk, using an orthogonal zsprayesi interface operating in both positive and negative ionisation modes. Uplcqtofms analysis of extracts from the leaves of pouteria. Intended use of the xevo g2xs qtof waters designed the orthogonal acceleration, timeofflight xevo g2xs qtof for use as a research tool to deliver authenticated mass measurement. A xevo g2s qtof waters, milford, ma, usa was used to obtain. Xevo g2 xs tof performance, robustness, and flexibility made accessible to everyone. Uplcqtofms analysis of extracts from the leaves of.
The xevo g2 xs qtof delivers not only conventional ms and msms methods of data acquisition, but also mse for comprehensive accurate mass precursor and fragment ion information from a single analysis, fastdda for rapid, intelligent automated accurate mass msms, and tof mrm for enhanced quantitative capability. Waters xevo g2 qtof xevo g2 qtof is the most sensitive, exactmass, quantitative and qualitative benchtop msms system available. In the ewre lab we have a watersmicromass xevo g2xs qtof, quadrupole timeofflight mass spectrometer. The instrument is developed to give the most accurate and reproducible separation of complex sample matrices. Overview to scale the picture below is a simulation at scale of the waters xevo g2xs qtof lcms mass spectrometer system right side connected with an acquity uhplc left side, installed on a ionbench ms mass spec bench or table. The waters xevo g2 qtof is coupled to a waters acquity uplc iclass, that offers the most powerful solutions to complex separation challenges. Xevo g2s tof instrument specifications 2 vacuum system differentially pumped, automated vacuum system comprising aircooled turbomolecular pumps and one backing pump either one rotary pump or one oil free pump. June 2011 720003448en ao pdf ms sensitivity esi the peak at mz 503 from a solution of 500 pgl raffinose in 7030 acetonitrilewater no additives, will have an intensity of greater than 1728 counts per second. Waters advances proteomics and lipidomics research with. Electroncapturedissociationofsodiumadductedpeptides on a. The xevo g2xs qtof mass spectrometer combines the new xs collision cell with the proven, signature technologies of tofmrm, stepwave tm.
Waters xevo g2 xs qtof manuals manuals and user guides for waters xevo g2 xs qtof. Xevo g2s tof is designed for scientists who need to identify and quantify the broadest range of compounds in complex and challenging samples. The xevo g2 xs tof combines stepwave ion optics, xs collision cell, and quantof technology for superior robustness, sensitivity, and selectivity along with dynamic range, mass accuracy, and speed of analysis, resulting in the highest quality, most comprehensive qualitative and quantitative information. All three platforms performed well with 90% of compounds detected in one set of spiked plasma samples, and 7988% for a second set. In the ewre lab we have a watersmicromass xevo g2 xs qtof, quadrupole timeofflight mass spectrometer.
519 1193 957 919 839 875 931 1121 1527 656 807 790 662 960 235 1043 1331 919 1194 1138 1469 1243 1512 880 1050 646 1133 337 1419 48 350 764 995 130 1292 210 633 548 670 166 391 47 1308 1371